Metabolomic Characterization of Italian Sweet Pepper (Capsicum annum L.) by Means of HRMAS-NMR Spectroscopy and Multivariate Analysis
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- 作者：Mena Ritota ; Federico Marini ; Paolo Sequi ; Massimiliano Valentini
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：September 8, 2010
- 年：2010
- 卷：58
- 期：17
- 页码：9675-9684
- 全文大小：935K
- 年卷期：v.58,no.17(September 8, 2010)
- ISSN：1520-5118
文摘
HRMAS-NMR spectroscopy was used to assess the metabolic profile of sweet pepper (Capsicum Annum L.). One-dimensional and two-dimensional NMR spectra, performed directly on sample pieces of few milligrams, hence without any chemical and/or physical manipulation, allowed the assignment of several compounds. Organic acids, fatty acids, amino acids, and minor compounds such as trigonelline, C4-substituted pyridine, choline, and cinnamic derivatives were observed with a single experiment. A significant discrimination between the two sweet pepper varieties was found by using partial least-squares projections to latent structures discrimination analysis (PLS-DA). The metabolites contributing predominantly to such differentiation were sugars and organic and fatty acids. Also a partial separation according to the geographical origin was obtained always by analyzing the NMR data with PLS-DA. Some of the discriminating molecules are peculiar for pepper and contribute to define the overall commercial and organoleptic quality so that HRMAS-NMR proved to be a complementary analysis to standard tools used in food science and, in principle, can be applied to any foodstuff.