Conjugation of Bioactive Ligands to PEG-Grafted Chitosan at the Distal End of PEG

详细信息    查看全文

• 作者：Eduardo Fernandez-Megia ; Ramó ; n Novoa-Carballal ; Emilio Qui&ntilde ; oá ; Ricardo Riguera
• 刊名：Biomacromolecules
• 出版年：2007
• 出版时间：March 2007
• 年：2007
• 卷：8
• 期：3
• 页码：833 - 842
• 全文大小：2148K
• 年卷期：v.8,no.3(March 2007)
• ISSN：1526-4602

文摘

Graft copolymers of chitosan and PEG-CO₂H incorporating biologically active molecules and tags (mannose,cholesterol, a coumarin dye, and biotin) at the distal end of poly(ethylene glycol) (PEG) have been synthesizedin excellent yields and nearly quantitative mass recoveries. Experimental conditions allowing the preparation ofmultifunctional graft copolymers incorporating simultaneously several of those active molecules and tags incontrolled ratios are also presented. The required functionalized PEG-CO₂H conjugates have been prepared froma heterodifunctional PEG and the experimental conditions established to ensure the purity of PEG end groups (¹Hand ¹³C NMR and matrix-assisted laser desorption/ionization mass spectrometry-time of flight (MALDI-TOF))and the completion of each synthetic step.