Maillard Reaction Products Modulating the Growth of Human Tumor Cells in Vitro
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- 作者：Doris Marko ; Michael Habermeyer ; Monika Kemé ; ny ; Ulrike Weyand ; Ellen Niederberger ; Oliver Frank ; and Thomas Hofmann
- 刊名：Chemical Research in Toxicology
- 出版年：2003
- 出版时间：January 2003
- 年：2003
- 卷：16
- 期：1
- 页码：48 - 55
- 全文大小：186K
- 年卷期：v.16,no.1(January 2003)
- ISSN：1520-5010
文摘
We investigated the effect of a series of Maillard reaction products formed from carbohydratesunder household heating conditions on the growth of human tumor cells in vitro. 4-Hydroxy-5-methyl-3-(2H)-furanone (1) was found to potently enhance the proliferation of human tumorcells. In contrast, the Maillard-type chromophores 2-(2-furyl)methylidene-4-hydroxy-5-methyl-2H-furan-3-one (2), 4-(2-furyl)-7-[(2-furyl)methylidene]-2-hydroxy-2H,7H,8aH-pyrano[2,3-b]pyran-3-one (6), and 3-hydroxy-4[(E)-(2-furyl)methylidene]methyl-3-cyclopentene-1,2 dione (13)inhibited the growth of human tumor cells in vitro in the low micromolar range. GXF251Lcells (gastric carcinoma), synchronized by serum deprivation, were retained in the G1-phaseof the cell cycle after treatment with 2, 6, or 13 for 24 h. Concomitantly, a distinct sub-G1peak was observed, indicative for apoptosis induction. DNA fragmentation was furtherinvestigated by ELISA using antibodies raised against histones and DNA. 2 induced asignificant increase of fragmented DNA at concentrations 
30 
M. After treatment withcompound 6, DNA fragmentation was observed at a higher concentration range (50 M),whereas incubation with 13 resulted in a marked DNA fragmentation already at 20 M. Onthe protein level, the activation of caspase 3, as an early marker for apoptosis induction, wasdetermined. The results were almost identical to those obtained in the DNA fragmentationELISA. In summary, Maillard reaction products potently modulating the growth of humantumor cells were identified. The Maillard-type chromophores 2, 6, and 13 were found to interferewith the proliferation of gastric carcinoma cells, causing cell cycle arrest and apoptosisinduction.
30 M. After treatment withcompound 6, DNA fragmentation was observed at a higher concentration range (50 M),whereas incubation with 13 resulted in a marked DNA fragmentation already at 20 M. Onthe protein level, the activation of caspase 3, as an early marker for apoptosis induction, wasdetermined. The results were almost identical to those obtained in the DNA fragmentationELISA. In summary, Maillard reaction products potently modulating the growth of humantumor cells were identified. The Maillard-type chromophores 2, 6, and 13 were found to interferewith the proliferation of gastric carcinoma cells, causing cell cycle arrest and apoptosisinduction.