Reduction of Active Elastase Concentration by Means of Immobilized Inhibitors: A Novel Therapeutic Approach
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- 作者：Valentina Grano ; Gianluca Tasco ; Rita Casadio ; Nadia Diano ; Marianna Portaccio ; Sergio Rossi ; Umberto Bencivenga ; Mario Compiani ; Anna De Maio ; and Damiano Gustavo Mita
- 刊名：Biotechnology Progress
- 出版年：2004
- 出版时间：June 2004
- 年：2004
- 卷：20
- 期：3
- 页码：968 - 974
- 全文大小：434K
- 年卷期：v.20,no.3(June 2004)
- ISSN：1520-6033
文摘
The inhibitory power of three different active Nylon membranes, separately loadedwith three different protease inhibitors, was studied with the aim of reducing theincreased elastase concentration occurring during hemodialysis or extracorporeal bloodcirculation in patients undergoing cardiopulmonary bypass. Chemical grafting wascarried out to make the inert Nylon membrane suitable for the immobilization of theinhibitors. The behavior of immobilized 
1-antitrypsin, bovine pancreatic trypsininhibitor (BPTI), or elastatinal was separately studied. 1-Antitrypsin and BPTI werecovalently immobilized by means of a diazotization process, whereas elastatinal wascovalently attached via a condensation process mediated by glutaraldehyde. Theinhibitory power of each membrane type was studied as a function of the amount ofimmobilized inhibitor and temperature. All active membranes have shown goodinhibitory power. The most efficient membrane was that loaded with 1-antitrypsin,the less efficient that with BPTI.
1-antitrypsin, bovine pancreatic trypsininhibitor (BPTI), or elastatinal was separately studied. 1-Antitrypsin and BPTI werecovalently immobilized by means of a diazotization process, whereas elastatinal wascovalently attached via a condensation process mediated by glutaraldehyde. Theinhibitory power of each membrane type was studied as a function of the amount ofimmobilized inhibitor and temperature. All active membranes have shown goodinhibitory power. The most efficient membrane was that loaded with 1-antitrypsin,the less efficient that with BPTI.