Enzyme Nanorings

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• 作者：Tsui-Fen Chou ; Christopher So ; Brian R. White ; Jonathan C. T. Carlson ; Mehmet Sarikaya ; Carston R. Wagner
• 刊名：ACS Nano
• 出版年：2008
• 出版时间：December 23, 2008
• 年：2008
• 卷：2
• 期：12
• 页码：2519-2525
• 全文大小：477K
• 年卷期：0
• ISSN：1936-086X

文摘

We have demonstrated that nanostructures, and in particular nanorings incorporating a homodimeric enzyme, can be prepared by chemically induced self-assembly of dihydrofolate reductase (DHFR)-histidine triad nucleotide binding 1 (Hint1) fusion proteins. The dimensions of the nanorings were found by static light scattering and atomic force microscopy studies to be dependent on the length and composition of the peptide linking the fusion proteins, ranging in size from 10 to 70 nm in diameter and 64 to 740 kDa. The catalytic efficiency of the nanorings was found to be dependent on ring size, thus suggesting that the arrangement of supermolecular assemblies of enzymes may be used to control their catalytic parameters.