Lectin from Phaseolus acutifolius var. escumite: Chemical Characterization, Sugar Specificity, and Effect on Human T-Lymphocytes
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- 作者：Adriana Castillo-Villanueva ; Heriberto Caballero-Ortega ; Fikrat Abdullaev- Jafarova ; Yonathan Garfias ; Maria del Carmen Jimé ; nez-Martí ; nez ; Sté ; phane Bouquelet ; Gisela Martí ; ne
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：July 11, 2007
- 年：2007
- 卷：55
- 期：14
- 页码：5781 - 5787
- 全文大小：167K
- 年卷期：v.55,no.14(July 11, 2007)
- ISSN：1520-5118
文摘
Purification of the lectin from Phaseolus acutifolius var. escumite was achieved by affinity chromatography on a column containing glutaraldehyzed membranes from blood group O erythrocytes. Thelectin is a tetrameric glycoprotein of 121 kDa with 10% of sugar by weight composed by four subunitsof 30 kDa as determined by SDS-PAGE. The lectin is composed of four isolectins as determined byion-exchange chromatography on a mono-S column. The lectin and its isolectins showed identicalNH2 terminal residues (ANDLSFNFQR FNETN) with homology to the PHA leucoagglutinin-precursor.Peptide mass fingerprint from each lectin isoform determined from tryptic peptides by MALDI-TOF(matrix assisted laser desorption ionization-time-of-flight) showed differences among subunits, thussuggesting microheterogeneity in their amino acid sequences or different glycosylation patterns. Thelectin and its four isolectins agglutinated erythrocytes without serological specificity and showedmitogenic activity on human leukocytes; moreover, the main effect was rather toward CD8+ than toCD4+ human peripheral lymphocytes. The lectin from escumite was not inhibitable by simple sugars;however, the specificity of the lectin and its isoforms was mainly addressed toward galactose residuespresent in bi- or triantennary N-acetyllactosamine-type glycans.