Isoelectric Point Determination of Norovirus Virus-like Particles by Capillary Isoelectric Focusing with Whole Column Imaging Detection
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- 作者：Lawrence Goodridge ; Carolyn Goodridge ; Jiaqi Wu ; Mansel Griffiths ; and Janusz Pawliszyn
- 刊名：Analytical Chemistry
- 出版年：2004
- 出版时间：January 1, 2004
- 年：2004
- 卷：76
- 期：1
- 页码：48 - 52
- 全文大小：114K
- 年卷期：v.76,no.1(January 1, 2004)
- ISSN：1520-6882
文摘
A capillary isoelectric focusing-whole column imagingdetection (CIEF-WCID) method was used to determinethe isoelectric point (pI) of norovirus virus-like particles(VLPs). The VLPs were produced from noroviruses thatrepresented the two genogroups, genogroup I (Funabashi,Seto, and Norwalk) and genogroup II (Hawaii, Kashiwa,and Narita). Using the imaged CIEF-WCID detectiontechnique, separation was accomplished using a short(4-5 cm) internally coated capillary (100-
m diameter)and a whole-column optical absorption imaging detectoroperated at 280 nm. CIEF-WCID experiments showed thesimilarity of the pI values of VLPs from genogroups I andII, with pI values of 5.9, 5.9, 6.0, and 6.0 for Funabashi,Norwalk, Seto, and Hawaii. The two other VLPs displayedpI values of 5.5 (Kashiwa) and 6.9 (Narita). The VLPpeaks were shown to be reproducibility resolved. CIEF-WCID shows great promise for norovirus detection inpublic health, clinical, and food safety applications, asCIEF-WCID overcomes several limitations of the currentlyused genetic and immunological methods.
m diameter)and a whole-column optical absorption imaging detectoroperated at 280 nm. CIEF-WCID experiments showed thesimilarity of the pI values of VLPs from genogroups I andII, with pI values of 5.9, 5.9, 6.0, and 6.0 for Funabashi,Norwalk, Seto, and Hawaii. The two other VLPs displayedpI values of 5.5 (Kashiwa) and 6.9 (Narita). The VLPpeaks were shown to be reproducibility resolved. CIEF-WCID shows great promise for norovirus detection inpublic health, clinical, and food safety applications, asCIEF-WCID overcomes several limitations of the currentlyused genetic and immunological methods.