Altering Adenoviral Tropism via Click Modification with ErbB Specific Ligands

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• 作者：Yoon Hyeun Oum ; Isaac S. Carrico
• 刊名：Bioconjugate Chemistry
• 出版年：2012
• 出版时间：July 18, 2012
• 年：2012
• 卷：23
• 期：7
• 页码：1370-1376
• 全文大小：332K
• 年卷期：v.23,no.7(July 18, 2012)
• ISSN：1520-4812

文摘

Methods for targeting oncolytic viruses can increase efficacy and accelerate development. Genetic engineering, the predominant method for changing vector tropism, is limited in scope and often represents the bottleneck for vector development. Metabolic incorporation of an unnatural azido sugar, O-GlcNAz, at a specific site on the adenoviral surface allows chemoselective attachment of affibodies for Her2 or EGF receptors. Modification with these high-affinity, high-selectivity proteins is straightforward and readily generalizable, demonstrates minimal impact on virus physiology, and affords significant increases in gene delivery to cancer cells. As a result, this method has significant potential to increase the efficacy of next-generation viral vectors.