β-Lactone Synthetase Found in the Olefin Biosynthesis Pathway
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- 作者：James K. ChristensonJack E. Richman ; Matthew R. Jensen ; Jennifer Y. Neufeld ; Carrie M. Wilmot ; Lawrence P. Wackett
- 刊名：Biochemistry
- 出版年：2017
- 出版时间：January 17, 2017
- 年：2017
- 卷：56
- 期：2
- 页码：348-351
- 全文大小：286K
- ISSN：1520-4995
文摘
The first β-lactone synthetase enzyme is reported, creating an unexpected link between the biosynthesis of olefinic hydrocarbons and highly functionalized natural products. The enzyme OleC, involved in the microbial biosynthesis of long-chain olefinic hydrocarbons, reacts with syn- and anti-β-hydroxy acid substrates to yield cis- and trans-β-lactones, respectively. Protein sequence comparisons reveal that enzymes homologous to OleC are encoded in natural product gene clusters that generate β-lactone rings, suggesting a common mechanism of biosynthesis.