Binding of EuIII to 1,2-Hydroxypyridinone-Modified Peptide Nucleic Acids

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• 作者：Arnie R. de Leon ; Abiola O. Olatunde ; Janet R. Morrow ; Catalina Achim
• 刊名：Inorganic Chemistry
• 出版年：2012
• 出版时间：December 3, 2012
• 年：2012
• 卷：51
• 期：23
• 页码：12597-12599
• 全文大小：202K
• 年卷期：v.51,no.23(December 3, 2012)
• ISSN：1520-510X

文摘

Substitution of a nucleobase pair with a pair of 1,2-hydroxypyridinone (1,2-HOPO) ligands in the center of a 10-base-pair peptide nucleic acid (PNA) duplex provides a strong binding site for Eu^III as evidenced by UV thermal melting curves, UV titrations, and luminescence spectroscopy. Eu^III excitation spectra and luminescence lifetime data are consistent with Eu^III bound to both 1,2 HOPO ligands in a PNA-HOPO duplex as the major species present in solution.