Anthocyanin pigments in the berries of bagua&
ccedil;u (
Eugenia umbelliflora Berg), a tropical fruit fromBrazil, were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified byAmberlite XAD-7 open-column chromatography. Six major anthocyanins were isolated by preparativeHPLC, and their chemical structures were identified by spectroscopic methods (TLC, UV-
vis, MS,and
1H NMR). Delphinidin 3-
O-
![](/images/gifchars/beta2.gif)
-glucopyranoside, cyanidin 3-
O-
![](/images/gifchars/beta2.gif)
-glucopyranoside, petunidin 3-
O-
![](/images/gifchars/beta2.gif)
-glucopyranoside, pelargonidin 3-
O-
![](/images/gifchars/beta2.gif)
-glucopyranoside, peonidin 3-
O-
![](/images/gifchars/beta2.gif)
-glucopyranoside, and mal
vidin3-
O-
![](/images/gifchars/beta2.gif)
-glucopyranoside were identified. On the basis of chromatographic data the total anthocyanincontent was 342 mg/100 g of fresh bagua&
ccedil;u berries. Therefore, the concomitant presence of sixanthocyanins in a single plant species makes this product promising as a new pigment source.Keywords:
Eugenia umbelliflora Berg; bagua&
ccedil;u; anthocyanins; natural colorants;
1H NMR