Synthesis of Collagen Nanotubes with Highly Regular Dimensions through Membrane-Templated Layer-by-Layer Assembly

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• 作者：Jessem Landoulsi ; Ccile J. Roy ; Christine Dupont-Gillain ; Sophie Demoustier-Champagne
• 刊名：Biomacromolecules
• 出版年：2009
• 出版时间：May 11, 2009
• 年：2009
• 卷：10
• 期：5
• 页码：1021-1024
• 全文大小：180K
• 年卷期：v.10,no.5(May 11, 2009)
• ISSN：1526-4602

文摘

Nanotubes made from a fibrillar protein, namely, collagen, were fabricated by a template-based method combined with layer-by-layer (LbL) deposition. The ability to incorporate collagen in LbL multilayered film was first demonstrated by in situ quartz crystal microbalance and ex situ ellipsometry on a flat model substrate, using poly(styrene sulfonate) (PSS) as polyanion. Collagen-based nanotubes were then fabricated by alternately immersing a polycarbonate membrane, used as template, in PSS and collagen aqueous solutions. Direct evidence for nanotube formation was obtained by dissolving the membrane and imaging the liberated (PSS/collagen)_n nanostructures by scanning electron microscopy and by transmission electron microscopy. The proposed strategy constitutes a practical alternative to electrospinning as it allows a very good control over the dimensions (outside and inside diameters and length) of the resulting nanotubes. Besides their fundamental interest, collagen-based nanotubes are useful nano-objects for the creation of new nanostructured biomaterials with numerous potential applications in the biomedical field.