ELISA as an Affordable Methodology for Monitoring Groundwater Contamination by Pesticides in Low-Income Countries

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• 作者：Beatriz M. Brena ; Lourdes Arellano ; Caterina Rufo ; Michael S. Last ; Jorge Montañ ; o ; Eduardo Egañ ; a Cerni ; Gualberto Gonzalez-Sapienza ; and Jerold A. Last
• 刊名：Environmental Science & Technology
• 出版年：2005
• 出版时间：June 1, 2005
• 年：2005
• 卷：39
• 期：11
• 页码：3896 - 3903
• 全文大小：133K
• 年卷期：v.39,no.11(June 1, 2005)
• ISSN：1520-5851

文摘

The traditional instrumental technology for pesticideresidue analysis is too expensive and labor-intense tomeet the regional needs concerning environmental monitoring.ELISA methodology was used for a pilot scale study ofgroundwater quality in an agricultural region a few kilometerssouthwest of Montevideo, the capital city of Uruguay.The study spanned 2 years and examined concentrations(detection limits are given in [ppb]) of two triazineherbicides (simazine [0.3] and atrazine [0.4]) and thecarbamate insecticide carbaryl [10] and its major metabolite1-naphthol [17]. In general, pesticide concentrationswere below detection limits in the samples tested and inall cases were well below the maximum contaminant levelsset by the U.S. EPA. 1-Naphthol was detected frequentlyby ELISA, but the assay may have tended to systematicallyoverestimate this analyte. To our knowledge, this is thefirst study of its type in Uruguay and perhaps the firstsystematic approach to monitoring for organic pesticidesin groundwater water sources in the temperate region ofSouth America.