The Nuclear Proteome of Chickpea (Cicer arietinum L.) Reveals Predicted and Unexpected Proteins

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• 作者：Aarti Pandey ; Mani Kant Choudhary ; Deepti Bhushan ; Arnab Chattopadhyay ; Subhra Chakraborty ; Asis Datta ; Niranjan Chakraborty
• 刊名：Journal of Proteome Research
• 出版年：2006
• 出版时间：December 2006
• 年：2006
• 卷：5
• 期：12
• 页码：3301 - 3311
• 全文大小：385K
• 年卷期：v.5,no.12(December 2006)
• ISSN：1535-3907

文摘

Nuclear proteins constitute a highly organized, complex network that plays diverse roles during cellulardevelopment and other physiological processes. The yeast nuclear proteome corresponds to aboutone-fourth of the total cellular proteins, suggesting the involvement of the nucleus in a number ofdiverse functions. In an attempt to understand the complexity of plant nuclear proteins, we havedeveloped a proteome reference map of a legume, chickpea, using two-dimensional gel electrophoresis(2-DE). Approximately, 600 protein spots were detected, and LC-ESI-MS/MS analyses led to theidentification of 150 proteins that have been implicated in a variety of cellular functions. The largestpercentage of the identified proteins was involved in signaling and gene regulation (36%), while 17%were involved in DNA replication and transcription. The chickpea nuclear proteome indicates thepresence of few new nuclear proteins of unknown functions vis-à-vis many known resident proteins.To the best of our knowledge, this is the first report of a nuclear proteome of an unsequenced genome.