Development of a Triplet–Triplet Absorption Ruler: DNA- and Chromatin-Mediated Drug Molecule Release from a Nanosurface

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• 作者：Sudeshna Das Chakraborty ; Abhishek Sau ; Denis V. Kuznetsov ; Amrita Banerjee ; Munmun Bardhan ; Maireyee Bhattacharya ; Dipak Dasgupta ; Samita Basu ; Dulal Senapati
• 刊名：Journal of Physical Chemistry B
• 出版年：2016
• 出版时间：July 14, 2016
• 年：2016
• 卷：120
• 期：27
• 页码：6872-6881
• 全文大小：772K
• 年卷期：0
• ISSN：1520-5207

文摘

Triplet–triplet (T–T) absorption spectroscopy has been used successfully as a molecular ruler to understand the actual release process of sanguinarine as a drug molecule from a gold nanoparticle surface in the presence of cell components, that is, DNA and chromatin. The obtained results have been verified by fluorescence and surface-enhanced Raman spectroscopy (SERS), and a plausible explanation has been put forward to describe the underestimation and overestimation of the percentage (%) of the release of drug molecules measured by fluorescence- and SERS-based techniques, respectively, over the highlighted T–T absorption spectroscopy. Because of the intrinsic nature of absorption, the reported T–T absorption spectroscopic assay overpowers fluorescence- and SERS-based assays, which are limited by the long-range interaction and nonlinear dependence of the concentration of analytes, respectively.