Separation, Identification, Quantification, and Method Validation of Anthocyanins in Botanical Supplement Raw Materials by HPLC and HPLC-MS
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- 作者：Amitabh Chandra ; Jatinder Rana ; and Yingqin Li
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2001
- 出版时间：August 2001
- 年：2001
- 卷：49
- 期：8
- 页码：3515 - 3521
- 全文大小：83K
- 年卷期：v.49,no.8(August 2001)
- ISSN：1520-5118
文摘
A method has been established and validated for identification and quantification of individual, aswell as total, anthocyanins by HPLC and LC/ES-MS in botanical raw materials used in the herbalsupplement industry. The anthocyanins were separated and identified on the basis of their respectiveM+ (cation) using LC/ES-MS. Separated anthocyanins were individually calculated against onecommercially available anthocyanin external standard (cyanidin-3-glucoside chloride) and expressedas its equivalents. Amounts of each anthocyanin calculated as external standard equivalent werethen multiplied by a molecular-weight correction factor to afford their specific quantities.Experimental procedures and use of a molecular-weight correction factors are substantiated andvalidated using Balaton tart cherry and elderberry as templates. Cyanidin-3-glucoside chloride hasbeen widely used in the botanical industry to calculate total anthocyanins. In our studies on tartcherry and elderberry, its use as external standard followed by use of molecular-weight correctionfactors should provide relatively accurate results for total anthocyanins, because of the presence ofcyanidin as their major anthocyanidin backbone. The method proposed here is simple and has adirect sample preparation procedure without any solid-phase extraction. It enables selection anduse of commercially available anthocyanins as external standards for quantification of specificanthocyanins in the sample matrix irrespective of their commercial availability as analyticalstandards. It can be used as a template and applied for similar quantification in several anthocyanin-containing raw materials for routine quality control procedures, thus providing consistency inanalytical testing of botanical raw materials used for manufacturing efficacious and true-to-the-label nutritional supplements.Keywords: Anthocyanins; ES-MS; Balaton tart cherry; Prunus cerasus L.; elderberry; Sambucusnigra L.; bilberry; Vaccinium myrtillus L.; chokeberry; Aronia melanocarpa Elliot; HPLC; LC-MS;botanical supplements; raw materials