High-Performance Liquid Chromatography Coupled with Coulometric Array Detection of Electroactive Components in Fruits and Vegetables: Relationship to Oxygen Radical Absorbance Capacity

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• 作者：Changjiang Guo ; Guohua Cao ; Emin Sofic ; and Ronald L. Prior
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1997
• 出版时间：May 1997
• 年：1997
• 卷：45
• 期：5
• 页码：1787 - 1796
• 全文大小：400K
• 年卷期：v.45,no.5(May 1997)
• ISSN：1520-5118

文摘

An HPLC procedure utilizing reversed-phase chromatography coupled with acoulometric arraydetection system was developed for the characterization of overallantioxidant status in fruits andvegetables. The method was reliable and sensitive (20 pg to 1 ngdetection limit) and can also beused to identify and simultaneously quantify multi-antioxidantsincluding vitamin C, glutathione,phenolic acids, and flavonoids in fruits and vegetables. Eachfruit and vegetable aqueous extractshowed an unique distribution of chromatographic peaks that could serveas a "fingerprint" for thefruit or vegetable. A significant positive linear correlation wasdemonstrated in fruit and vegetableaqueous extracts between the total antioxidant activities determined byusing oxygen radicalabsorbance capacity (ORAC_ROO)assay and the electrochemical data generated from thecoulometricarray detectors. From these data, theORAC_ROO assay appears to be avalid and useful procedurefor measuring total antioxidant activity in extracts of fruits andvegetables.Keywords: Fruit; vegetable; antioxidant; phenolic acid; flavonoid;anthocyanin