文摘
In this study, a mat/membrane consisting of overlaid PVA/PA6鈥揅u(II) composite nanofibers was prepared via the electrospinning technique followed by coordination/chelation with Cu(II) ions; an enzyme of catalase (CAT) was then immobilized onto the PVA/PA6鈥揅u(II) nanofibrous membrane. The amount of immobilized catalase reached a high value of 64 卤 4.6 mg/g, while the kinetic parameters (Vmax and Km) of enzyme were 3774 渭mol/mg路min and 41.13 mM, respectively. Furthermore, the thermal stability and storage stability of immobilized catalase were improved significantly. Thereafter, a plug-flow type of immobilized enzyme membrane reactor (IEMR) was assembled from the PVA/PA6鈥揅u(II)鈥揅AT membrane. With the increase of operational pressure from 0.02 to 0.2 MPa, the flux value of IEMR increased from 0.20 卤 0.02 to 0.76 卤 0.04 L/m2路min, whereas the conversion ratio of H2O2 decreased slightly from 92 卤 2.5% to 87 卤 2.1%. After 5 repeating cycles, the production capacity of IEMR was merely decreased from 0.144 卤 0.006 to 0.102 卤 0.004 mol/m2路min. These results indicated that the assembled IEMR possessed high productivity and excellent reusability, suggesting that the IEMR based on electrospun PVA/PA6鈥揅u(II) nanofibrous membrane might have great potential for various applications, particularly those related to environmental protection.