Elucidating Hydroxylation and Methylation Steps Tailoring Piericidin A1 Biosynthesis

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• 作者：Yaolong Chen ; Wenjun Zhang ; Yiguang Zhu ; Qingbo Zhang ; Xinpeng Tian ; Si Zhang ; Changsheng Zhang
• 刊名：Organic Letters
• 出版年：2014
• 出版时间：February 7, 2014
• 年：2014
• 卷：16
• 期：3
• 页码：736-739
• 全文大小：256K
• ISSN：1523-7052

文摘

The piericidin A1 (1) gene cluster was identified from the deep-sea derived Streptomyces sp. SCSIO 03032. Our in vivo and in vitro experiments verified PieE as a 4鈥?hydroxylase and PieB2 as a 4鈥?O-methyltransferase, allowing the elucidation of the post-PKS modification steps involved in 1 biosynthesis. In addition, the shunt metabolite piericidin E1 (7) was identified as a novel analogue featuring a C-2/C-3 epoxy ring.