Recognition of Proteins by Crystallization Patterns in an Array of Reporter Solution Microdroplets

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• 作者：Victor N. Morozov ; Nikolai N. Vsevolodov ; Adam Elliott ; and Charles Bailey
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：January 1, 2006
• 年：2006
• 卷：78
• 期：1
• 页码：258 - 264
• 全文大小：607K
• 年卷期：v.78,no.1(January 1, 2006)
• ISSN：1520-6882

文摘

A new technique is described for specific recognition ofprotein analytes by observing protein-induced changes inthe drying/crystallization patterns (DCP) of an array ofmicrodroplets containing solutions of different reportersubstances. Recognition is based on a difference ininteraction of the protein analyte crystalline elements(planes, edges, defects, etc.) in the growing reportercrystals. Using a set of natural L-amino acids as reportersand denoting the amino acid solutions displaying substantial protein-induced changes in the DCP as "1" andthose that show no or small changes as "0", a digitalbinary code was determined for several proteins atmultiple concentrations. It was demonstrated that globular proteins can be reliably identified using this code as a"signature" when only 2-100 ng of protein was added toamino acid microdroplets.