Photoactivatable Cross-Linked Polyacrylamide for the Site-Selective Immobilization of Antigens and Antibodies
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- 作者：Melanie S. Sanford ; Paul T. Charles ; Sarah M. Commisso ; Jenna C. Roberts ; and David W. Conrad
- 刊名：Chemistry of Materials
- 出版年：1998
- 出版时间：June 1998
- 年：1998
- 卷：10
- 期：6
- 页码：1510 - 1520
- 全文大小：321K
- 年卷期：v.10,no.6(June 1998)
- ISSN：1520-5002
文摘
This paper describes the synthesis, characterization,and photochemical behavior of a newlight-activated material for the photoimmobilization of antigens.The material is a derivativeof cross-linked polyacrylamide that incorporates photoactiveo-nitrobenzyl carbamates. Whenirradiated, the polymer undergoes a photochemical rearrangement toproduce primary aminesthat can be used as molecular attachment sites. We monitored thephotoconversion of thin(1-2 
m) polymer films that were deposited on silicon wafers orfused silica substrates usingFT IR spectroscopy and UV-vis spectroscopy. To produce patternedpolymer-modifiedsubstrates, we irradiated the material using a photolithographic mask.This process yielded10-m lines of photogenerated amines, to which an amine-reactiveantigen (2,4,6-trintrobenzenesulfonic acid) was covalently bound. When we used thisantigen-patterned substratein a competitive fluorescence immunoassay containingtetramethylrhodamine-labeled anti-2,4-dinitrophenyl antibodies and 2,4-dinitrophenol, concentrations of2,4-dinitrophenol aslow as 2.3 g/mL were detectable.
m) polymer films that were deposited on silicon wafers orfused silica substrates usingFT IR spectroscopy and UV-vis spectroscopy. To produce patternedpolymer-modifiedsubstrates, we irradiated the material using a photolithographic mask.This process yielded10-m lines of photogenerated amines, to which an amine-reactiveantigen (2,4,6-trintrobenzenesulfonic acid) was covalently bound. When we used thisantigen-patterned substratein a competitive fluorescence immunoassay containingtetramethylrhodamine-labeled anti-2,4-dinitrophenyl antibodies and 2,4-dinitrophenol, concentrations of2,4-dinitrophenol aslow as 2.3 g/mL were detectable.