Effects of Oxygen on Recombinant Protein Expression

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• 作者：John O. Konz ; Jonathan King ; and Charles L. Cooney
• 刊名：Biotechnology Progress
• 出版年：1998
• 出版时间：June 1998
• 年：1998
• 卷：14
• 期：3
• 页码：393 - 409
• 全文大小：269K
• 年卷期：v.14,no.3(June 1998)
• ISSN：1520-6033

文摘

Efforts to increase cell growth and protein yields need to becomplemented by themaintenance of the quality of the protein produced. Elevatedoxygen pressure or rapidincreases in oxygen content can cause oxidative stress within thecells, leading tooxidation of specific proteins and nucleotide sequences. Inaddition, transient orsteady-state anoxic conditions can cause limitations in amino acidproduction andplasmid stability. Major pathways and mechanisms of oxidativedamage to proteinsexpressed in bacteria are reviewed. Damage to nucleic acidsinvolved in geneexpression also is considered. The methodologies for identifyingoxidative damage tomacromolecules are improving but are not yet adequate for on-linefeedback. Thislimits our ability to integrate information about these phenomena andthe cellularresponses into a quantitative model. Enough information isavailable, however, toconsider changes in the time profile of dissolved oxygen as a cause forpoor processperformance.