Microbial Incorporation of 13C-Labeled Acetate at the Field Scale: Detection of Microbes Responsible for Reduction of U(VI)
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- 作者：Yun-Juan Chang ; Philip E. Long ; Roland Geyer ; Aaron D. Peacock ; Charles T. Resch ; Kerry Sublette ; Susan Pfiffner ; Amanda Smithgall ; Robert T. Anderson ; Helen A. Vrionis ; John R.Stephen ; Richard Dayvau
- 刊名：Environmental Science & Technology
- 出版年：2005
- 出版时间：December 1, 2005
- 年：2005
- 卷：39
- 期：23
- 页码：9039 - 9048
- 全文大小：299K
- 年卷期：v.39,no.23(December 1, 2005)
- ISSN：1520-5851
文摘
A field-scale acetate amendment experiment wasperformed in a contaminated aquifer at Old Rifle, CO tostimulate in situ microbial reduction of U(VI) in groundwater.To evaluate the microorganisms responsible for microbialuranium reduction during the experiment, 13C-labeledacetate was introduced into well bores via bio-traps containingporous activated carbon beads (Bio-Sep). Incorporationof the 13C from labeled acetate into cellular DNA andphospholipid fatty acid (PLFA) biomarkers was analyzed inparallel with geochemical parameters. An enrichment ofactive 
ta.gif" BORDER=0 >-proteobacteria was demonstrated in downgradientmonitoring wells: Geobacter dominated in wells closerto the acetate injection gallery, while various sulfate reducerswere prominent in different downgradient wells. Theseresults were consistent with the geochemical evidence ofFe(III), U(VI), and SO42- reduction. PLFA profiling of bio-traps suspended in the monitoring wells also showed theincorporation of 13C into bacterial cellular lipids. Communitycomposition of downgradient monitoring wells basedon quinone and PLFA profiling was in general agreementwith the 13C-DNA result. The direct application of 13C labelto biosystems, coupled with DNA and PLFA analysis,which combined detailed taxonomic description with aquantitative measure of metabolic diversity, allowedidentification of the metabolically active portion of themicrobial community during reduction of U(VI).
ta.gif" BORDER=0 >-proteobacteria was demonstrated in downgradientmonitoring wells: Geobacter dominated in wells closerto the acetate injection gallery, while various sulfate reducerswere prominent in different downgradient wells. Theseresults were consistent with the geochemical evidence ofFe(III), U(VI), and SO42- reduction. PLFA profiling of bio-traps suspended in the monitoring wells also showed theincorporation of 13C into bacterial cellular lipids. Communitycomposition of downgradient monitoring wells basedon quinone and PLFA profiling was in general agreementwith the 13C-DNA result. The direct application of 13C labelto biosystems, coupled with DNA and PLFA analysis,which combined detailed taxonomic description with aquantitative measure of metabolic diversity, allowedidentification of the metabolically active portion of themicrobial community during reduction of U(VI).