Hybrid Aptamer-Antibody Linked Fluorescence Resonance Energy Transfer Based Detection of Trinitrotoluene

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• 作者：Priyanka Sabherwal ; Munish Shorie ; Preeti Pathania ; Shilpa Chaudhary ; K. K. Bhasin ; Vijayender Bhalla ; C. Raman Suri
• 刊名：Analytical Chemistry
• 出版年：2014
• 出版时间：August 5, 2014
• 年：2014
• 卷：86
• 期：15
• 页码：7200-7204
• 全文大小：429K
• ISSN：1520-6882

文摘

Combining synthetic macromolecules and biomolecular recognition units are promising in developing novel diagnostic and analysis techniques for detecting environmental and/or clinically important substances. Fluorescence resonance energy transfer (FRET) apta-immunosensor for explosive detection is reported using 2,4,6-trinitrotoluene (TNT) specific aptamer and antibodies tagged with respective FRET pair dyes in a sandwich immunoassay format. FITC-labeled aptamer was used as a binder molecule in the newly developed apta-immunoassay format where the recognition element was specific anti-TNT antibody labeled with rhodamine isothiocyanate. The newly developed sensing platform showed excellent sensitivity with a detection limit of the order of 0.4 nM presenting a promising candidate for routine screening of TNT in samples.