Fast Isoelectric Focusing and Antipeptide Antibodies for Detecting Bovine Casein in Adulterated Water Buffalo Milk and Derived Mozzarella Cheese

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• 作者：Francesco Addeo ; Rosa Pizzano ; Maria Adalgisa Nicolai ; Simonetta Caira ; Lina Chianese
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：November 11, 2009
• 年：2009
• 卷：57
• 期：21
• 页码：10063-10066
• 全文大小：696K
• 年卷期：v.57,no.21(November 11, 2009)
• ISSN：1520-5118

文摘

Plasmin hydrolysis of water buffalo casein (CN) can liberate a peptide comigrating with bovine γ₂-CN. Occurrence of this peptide may lead to false-positive detection of cow’s milk for a genuine water buffalo cheese when it is analyzed by applying a fast version of the European official method for detecting bovine casein in water buffalo cheese. After isoelectric focusing of CN plasminolysates, performed according to the official method, immunoblot analysis with antipeptide antibodies was assayed to distinguish between γ₂-CN and the interfering bovine γ₂-CN-like peptide. Small, synthetic peptides containing partial sequences of bovine γ₂-CN were used as immunogens for antipeptide antibodies raised in rabbits. The antibody preparation directed toward the synthetic peptide containing the first five amino acid residues of γ₂-CN cross-reacted with native and in vitro generated γ₂-CN from bovine and water buffalo CN, but it did not recognize the bovine γ₂-CN-like band in the electrophoretic profile of pure water buffalo CN.