Hybridization of Pulsed-Q Dissociation and Collision-Activated Dissociation in Linear Ion Trap Mass Spectrometer for iTRAQ Quantitation

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• 作者：Tiannan Guo ; Chee Sian Gan ; Huoming Zhang ; Yi Zhu ; Oi Lian Kon ; Siu Kwan Sze
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：November 7, 2008
• 年：2008
• 卷：7
• 期：11
• 页码：4831-4840
• 全文大小：500K
• 年卷期：v.7,no.11(November 7, 2008)
• ISSN：1535-3907

文摘

Coupling of multiplex isobaric tags for relative and absolute quantitation (iTRAQ) to a sensitive linear ion trap (LTQ) mass spectrometer (MS) is a challenging, but highly promising approach for quantitative high-throughput proteomic profiling. Integration of the advantages of pulsed-Q dissociation (PQD) and collision-activated dissociation (CAD) fragmentation methods into a PQD−CAD hybrid mode, together with PQD optimization and data manipulation with a bioinformatics algorithm, resulted in a robust, sensitive and accurate iTRAQ quantitative proteomic workflow. The workflow was superior to the default PQD setting when profiling the proteome of a gastric cancer cell line, SNU5. Taken together, we established an optimized PQD-CAD hybrid workflow in LTQ-MS for iTRAQ quantitative proteomic profiling that may have wide applications in biological and biomedical research.