Carbohydrate Analysis Experiment Involving Mono- and Disaccharides with a Twist of Glycobiology: Two New Tests for Distinguishing Pentoses and Glycosidic Bonds
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- 作者：Chelsea Herdman ; Lamine Diop ; Michael Dickman
- 刊名：Journal of Chemical Education
- 出版年：2013
- 出版时间：January 8, 2013
- 年：2013
- 卷：90
- 期：1
- 页码：115-117
- 全文大小：158K
- 年卷期：v.90,no.1(January 8, 2013)
- ISSN：1938-1328
文摘
Carbohydrate analysis is an excellent way to expose second-year biochemistry majors to the subtlety and nuance of sugar chemistry. In one, 3-h practical period, students must identify an unknown mono- or disaccharide from a collection of three hexoses (glucose, galactose, and mannose), two pentoses (ribose and arabinose), and three disaccharides (maltose, cellobiose, and lactose). Two new tests have been developed: an enzyme assay with 伪-glucosidase followed by the Barfoed test quickly distinguishes maltose and cellobiose and 1,1-diphenylhydrazine is used to identify arabinose. The exercise is presented as a way to sensitize new students of biochemistry to the communicative aspect of sugars.