A Homogeneous Chemiluminescent Immunoassay Method
详细信息 查看全文
- 作者：Hashem Akhavan-Tafti ; Dean G. Binger ; John J. Blackwood ; Ying Chen ; Richard S. Creager ; Renuka de Silva ; Robert A. Eickholt ; Jose E. Gaibor ; Richard S. Handley ; Kenneth P. Kapsner ; Senja K. Lopac ; Michael E. Mazelis ; Terri L. McLernon ; James D. Mendoza ; Bruce H. Odegaard ; Sarada G. Reddy ; Michael Salvati ; Barry A. Schoenfelner ; Nir Shapir ; Katherine R. Shelly ; Jeff C. Todtleben ; Guoping Wang ; Wenhua Xie
- 刊名：The Journal of the American Chemical Society
- 出版年：2013
- 出版时间：March 20, 2013
- 年：2013
- 卷：135
- 期：11
- 页码：4191-4194
- 全文大小：244K
- 年卷期：v.135,no.11(March 20, 2013)
- ISSN：1520-5126
文摘
A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trigger solution, and the intensity is directly correlated to the quantity of the analyte.