Induction of Apoptosis by [8]-Shogaol via Reactive Oxygen Species Generation, Glutathione Depletion, and Caspase Activation in Human Leukemia Cells

详细信息    查看全文

• 作者：Po-Chuen Shieh ; Yi-Own Chen ; Daih-Huang Kuo ; Fu-An Chen ; Mei-Ling Tsai ; Ing-Shing Chang ; Hou Wu ; Shengmin Sang ; Chi-Tang Ho ; Min-Hsiung Pan
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：March 24, 2010
• 年：2010
• 卷：58
• 期：6
• 页码：3847-3854
• 全文大小：1006K
• 年卷期：v.58,no.6(March 24, 2010)
• ISSN：1520-5118

文摘

Ginger, the rhizome of Zingiber officinale, is a traditional medicine with a carminative effect and antinausea, anti-inflammatory, and anticarcinogenic properties. This study examined the growth inhibitory effects of [8]-shogaol, one of the pungent phenolic compounds in ginger, on human leukemia HL-60 cells. It demonstrated that [8]-shogaol was able to induce apoptosis in a time- and concentration-dependent manner. Treatment with [8]-shogaol caused a rapid loss of mitochondrial transmembrane potential, stimulation of reactive oxygen species (ROS) production, release of mitochondrial cytochrome c into cytosol, and subsequent induction of procaspase-9 and procaspase-3 processing. Taken together, these results suggest for the first time that ROS production and depletion of glutathione that contributed to [8]-shogaol-induced apoptosis in HL-60 cells.