Building Blocks for the Development of an Interface for High-Throughput Thin Layer Chromatography/Ambient Mass Spectrometric Analysis: A Green Methodology

详细信息    查看全文

• 作者：Sy-Chyi Cheng ; Min-Zong Huang ; Li-Chieh Wu ; Chih-Chiang Chou ; Chu-Nian Cheng ; Siou-Sian Jhang ; Jentaie Shiea
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：July 17, 2012
• 年：2012
• 卷：84
• 期：14
• 页码：5864-5868
• 全文大小：352K
• 年卷期：v.84,no.14(July 17, 2012)
• ISSN：1520-6882

文摘

Interfacing thin layer chromatography (TLC) with ambient mass spectrometry (AMS) has been an important area of analytical chemistry because of its capability to rapidly separate and characterize the chemical compounds. In this study, we have developed a high-throughput TLC鈥揂MS system using building blocks to deal, deliver, and collect the TLC plate through an electrospray-assisted laser desorption ionization (ELDI) source. This is the first demonstration of the use of building blocks to construct and test the TLC鈥揗S interfacing system. With the advantages of being readily available, cheap, reusable, and extremely easy to modify without consuming any material or reagent, the use of building blocks to develop the TLC鈥揂MS interface is undoubtedly a green methodology. The TLC plate delivery system consists of a storage box, plate dealing component, conveyer, light sensor, and plate collecting box. During a TLC鈥揂MS analysis, the TLC plate was sent to the conveyer from a stack of TLC plates placed in the storage box. As the TLC plate passed through the ELDI source, the chemical compounds separated on the plate would be desorbed by laser desorption and subsequently postionized by electrospray ionization. The samples, including a mixture of synthetic dyes and extracts of pharmaceutical drugs, were analyzed to demonstrate the capability of this TLC鈥揈LDI/MS system for high-throughput analysis.