Synthesis and Evaluation of a New Fluorescent Transglycosylase Substrate: Lipid II-Based Molecule Possessing a Dansyl-C20 Polyprenyl Moiety
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- 作者：Chen-Yu Liu ; Chih-Wei Guo ; Yi-Fan Chang ; Jen-Tsung Wang ; Hao-Wei Shih ; Yu-Fang Hsu ; Chia-Wei Chen ; Shao-Kang Chen ; Yen-Chih Wang ; Ting-Jen R. Cheng ; Che Ma ; Chi-Huey Wong ; Jim-Min Fang ; Wei-Chieh Cheng
- 刊名：Organic Letters
- 出版年：2010
- 出版时间：April 2, 2010
- 年：2010
- 卷：12
- 期：7
- 页码：1608-1611
- 全文大小：233K
- 年卷期：v.12,no.7(April 2, 2010)
- ISSN：1523-7052
文摘
The preparation of a novel fluorescent lipid II-based substrate for transglycosylases (TGases) is described. This substrate has characteristic structural features including a shorter lipid chain, a fluorophore tag at the end of the lipid chain rather than on the peptide chain, and no labeling with a radioactive atom. This fluorescent substrate is readily utilized in TGase activity assays to characterize TGases and also to evaluate the activities of TGase inhibitors.