Comparative Proteomic Analysis of Indioside D-Triggered Cell Death in HeLa Cells

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• 作者：Chi Chun Wong ; Ying Wang ; Ka-Wing Cheng ; Jen-Fu Chiu ; Qing-Yu He ; Feng Chen
• 刊名：Journal of Proteome Research
• 出版年：2008
• 出版时间：May 2008
• 年：2008
• 卷：7
• 期：5
• 页码：2050 - 2058
• 全文大小：5263K
• 年卷期：v.7,no.5(May 2008)
• ISSN：1535-3907

文摘

Medicinal plants represent a rich source of cancer drug leads. Indioside D, a furostanol glycoside isolated from Solanum mammosum, was found to possess antiproliferative activity toward a panel of human cancer cell lines. Proteomic analysis of indioside D-treated HeLa cells revealed profound protein changes related to energy production and oxidative stress, suggesting that mitochondria dysfunction plays a role in indioside D-induced apoptosis. Indioside D caused a rapid dissipation of mitochondrial transmembrane potential (ΔΨ_m) and the generation of reactive oxygen species (ROS), leading to the activation of caspase-dependent apoptotic cell death. The Fas death receptor pathway was also activated following indioside D treatment, and triggered the activation of caspase-8 and cleavage of Bid, which also acted through the mitochondrial apoptosis pathway. These results suggest that indioside D induced apoptosis in HeLa cells via both intrinsic and extrinsic cell death pathways.