Surface Properties of "Jellyfish": Langmuir Monolayer and Langmuir-Blodgett Film Studies of Recombinant Aequorin
详细信息 查看全文
- 作者：Chengshan Wang ; Miodrag Micic ; Mark Ensor ; Sylvia Daunert ; Roger M. Leblanc
- 刊名：Langmuir
- 出版年：2007
- 出版时间：July 3, 2007
- 年：2007
- 卷：23
- 期：14
- 页码：7602 - 7607
- 全文大小：113K
- 年卷期：v.23,no.14(July 3, 2007)
- ISSN：1520-5827
文摘
In this paper, we studied the surface properties of recombinant aequorin at the air-water interface. Using theLangmuir monolayer technique, the surface properties of aequorin were studied, including the surface pressure andsurface potential-area isotherms, compression-decompression cycles, and stability on Trizma Base (Tris/HCl) bufferat pH 7.6. The results showed that aequorin formed a stable Langmuir monolayer and the surface pressure-areaisotherms were dependent on both pH and ionic strength. At a pH higher or lower than 7.6, the limiting moleculararea decreased. The circular dichroism (CD) spectra of aequorin in aqueous solutions explained this result: when thepH was higher than 7.6, the 
-helix conformation changed to unordered structures, whereas at a pH lower than 7.6,the -helix conformation changed to 
-sheet. The addition of calcium chloride to the Tris/HCl buffer subphase (pH7.6) caused an increase of the limiting molecular area of the aequorin Langmuir monolayer. The fluorescence spectraof a Langmuir-Blodgett (LB) film of aequorin in the presence of calcium chloride indicated that the aequorintransformed to the apoaequorin.
-helix conformation changed to unordered structures, whereas at a pH lower than 7.6,the -helix conformation changed to -sheet. The addition of calcium chloride to the Tris/HCl buffer subphase (pH7.6) caused an increase of the limiting molecular area of the aequorin Langmuir monolayer. The fluorescence spectraof a Langmuir-Blodgett (LB) film of aequorin in the presence of calcium chloride indicated that the aequorintransformed to the apoaequorin.