Glionitrin A, an Antibiotic−Antitumor Metabolite Derived from Competitive Interaction between Abandoned Mine Microbes

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• 作者：Hyun Bong Park^† ; ; Hak Cheol Kwon*^† ; ; Chang-Ho Lee^‡ ; ; Hyun Ok Yang^† ;
• 刊名：Journal of Natural Products
• 出版年：2009
• 出版时间：February 27, 2009
• 年：2009
• 卷：72
• 期：2
• 页码：248-252
• 全文大小：141K
• 年卷期：v.72,no.2(February 27, 2009)
• ISSN：1520-6025

文摘

The nutrient conditions present in abandoned coal mine drainages create an extreme environment where defensive and offensive microbial interactions could be critical for survival and fitness. Coculture of a mine drainage-derived Sphingomonas bacterial strain, KMK-001, and a mine drainage-derived Aspergillus fumigatus fungal strain, KMC-901, resulted in isolation of a new diketopiperazine disulfide, glionitrin A (1). Compound 1 was not detected in monoculture broths of KMK-001 or KMC-901. The structure of 1, a (3S,10aS) diketopiperazine disulfide containing a nitro aromatic ring, was based on analysis of MS, NMR, and circular dichroism spectra and confirmed by X-ray crystal data. Glionitrin A displayed significant antibiotic activity against a series of microbes including methicillin-resistant Staphylococcus aureus. An in vitro MTT cytotoxicity assay revealed that 1 had potent submicromolar cytotoxic activity against four human cancer cell lines: HCT-116, A549, AGS, and DU145. The results provide further evidence that microbial coculture can produce novel biologically relevant molecules.