Anti-inflammatory Activity of Saxifragin via Inhibition of NF-魏B Involves Caspase-1 Activation

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• 作者：Se-Yun Cheon ; Kyung-Sook Chung ; Eunjin Jeon ; Agung Nugroho ; Hee-Jun Park ; Hyo-Jin An
• 刊名：Journal of Natural Products
• 出版年：2015
• 出版时间：July 24, 2015
• 年：2015
• 卷：78
• 期：7
• 页码：1579-1585
• 全文大小：469K
• ISSN：1520-6025

文摘

Saxifragin, the 5-glucoside of the flavonoid quercetin, is found in plants and insects. It has been reported that saxifragin has peroxynitrite-scavenging effects. However, the mechanism of anti-inflammatory effects of saxifragin has not yet been clearly identified. In this study, we investigated the anti-inflammatory effects of saxifragin in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and animal models of inflammation. We found that saxifragin suppressed the production of nitric oxide (NO) and prostaglandin E₂ (PGE₂) in LPS-activated RAW 264.7 macrophages by suppressing the level of protein and mRNA expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively. Furthermore, saxifragin inhibited mRNA expression of pro-inflammatory cytokines including tumor necrosis factor (TNF)-伪, interleukin (IL)-6, and IL-1尾. We studied the inhibitory effects of saxifragin on the nuclear translocation of nuclear factor (NF)-魏B, activation of caspase-1, and phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). Furthermore, pretreatment with saxifragin increased the survival rate of mice with LPS-induced septic death. Collectively, these findings suggest that saxifragin exerts anti-inflammatory activity by inhibiting NF-魏B, caspase-1, and mitogen-activated protein kinase (MAPK) activation.