Development of High-Sensitivity Ion Trap Ion Mobility Spectrometry Time-of-Flight Techniques: A High-Throughput Nano-LC-IMS-TOF Separation of Peptides Arising from a Drosophila Protein Extract

详细信息    查看全文

• 作者：Sunnie Myung ; Young Jin Lee ; Myeong Hee Moon ; John Taraszka ; Rena Sowell ; Stormy Koeniger ; Amy E. Hilderbrand ; Stephen J. Valentine ; Lucy Cherbas ; Peter Cherbas ; Thomas C. Kaufmann ; David F. Miller ; Y
• 刊名：Analytical Chemistry
• 出版年：2003
• 出版时间：October 1, 2003
• 年：2003
• 卷：75
• 期：19
• 页码：5137 - 5145
• 全文大小：612K
• 年卷期：v.75,no.19(October 1, 2003)
• ISSN：1520-6882

文摘

A linear octopole trap interface for an ion mobility time-of-flight mass spectrometer has been developed for focusing and accumulating continuous beams of ions producedby electrospray ionization. The interface improves experimental efficiencies by factors of ~50-200 compared withan analogous configuration that utilizes a three-dimensional Paul geometry trap (Hoaglund-Hyzer, C. S.; Lee,Y. J.; Counterman, A. E.; Clemmer, D. E. Anal. Chem.2002, 74, 992-1006). With these improvements, it ispossible to record nested drift (flight) time distributionsfor complex mixtures in fractions of a second. We demonstrate the approach for several well-defined peptidemixtures and an assessment of the detection limits isgiven. Additionally, we demonstrate the utility of theapproach in the field of proteomics by an on-line, three-dimensional nano-LC-ion mobility-TOF separation of tryptic peptides from the Drosophila proteome.