A Bioaccumulative Cyclometalated Platinum(II) Complex with Two-Photon-Induced Emission for Live Cell Imaging
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- 作者：Chi-Kin Koo ; Ka-Leung Wong ; Cornelia Wing-Yin Man ; Yun-Wah Lam ; Leo King-Yan So ; Hoi-Lam Tam ; Sai-Wah Tsao ; Kok-Wai Cheah ; Kai-Chung Lau ; Yang-Yi Yang ; Jin-Can Chen ; Michael Hon-Wah Lam
- 刊名：Inorganic Chemistry
- 出版年：2009
- 出版时间：February 2, 2009
- 年：2009
- 卷：48
- 期：3
- 页码：872-878
- 全文大小：438K
- 年卷期：v.48,no.3(February 2, 2009)
- ISSN：1520-510X
文摘
The cyclometalated platinum(II) complex [Pt(L)Cl], where HL is a new cyclometalating ligand 2-phenyl-6-(1H-pyrazol-3-yl)pyridine containing Cphenyl, Npyridyl, and Npyrazolyl donor moieties, was found to possess two-photon-induced luminescent properties. The two-photon-absorption cross section of the complex in N,N-dimethylformamide at room temperature was measured to be 20.8 GM. Upon two-photon excitation at 730 nm from a Ti:sapphire laser, bright-green emission was observed. Besides its two-photon-induced luminescent properties, [Pt(L)Cl] was able to be rapidly accumulated in live HeLa and NIH3T3 cells. The two-photon-induced luminescence of the complex was retained after live cell internalization and can be observed by two-photon confocal microscopy. Its bioaccumulation properties enabled time-lapse imaging of the internalization process of the dye into living cells. Cytotoxicity of [Pt(L)Cl] to both tested cell lines was low, according to MTT assays, even at loadings as high as 20 times the dose concentration for imaging for 6 h.