Direct Synthesis of Rev Peptide-Conjugated Gold Nanoparticles and Their Application in Cancer Therapeutics

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• 作者：Ngoc Thi Thanh Tran ; Tzu-Hao Wang ; Chiao-Yun Lin ; Yi-Chun Tsai ; Chyong-Huey Lai ; Yian Tai ; Benjamin ; Y. M. Yung
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：July 20, 2011
• 年：2011
• 卷：22
• 期：7
• 页码：1394-1401
• 全文大小：973K
• 年卷期：v.22,no.7(July 20, 2011)
• ISSN：1520-4812

文摘

We have developed a simple approach for generating peptide-conjugated gold nanoparticles (AuNPs) from the Rev peptide and gold aqueous solution. The peptide functions as both a reducing agent and a capping molecule. AuNPs of various sizes (20鈥?00 nm) and shapes (spheres, triangular plates, and polygons) can be obtained upon modulating the ratio of gold ions to the Rev peptide. Transmission electron microscopy, X-ray diffraction, and UV鈥搗is spectroscopy were utilized to characterize these nanoparticles. Fourier-transform infrared and X-ray photoelectron spectroscopy measurements were performed to investigate chemical interactions between the Rev peptide and AuNPs. Lactate dehydrogenase and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays revealed that the Rev peptide鈥揂uNP nanocomposites exhibited exceptionally high cytotoxic effects toward mouse ovarian surface epithelial cell lines, relative to the effects of equal doses of the free Rev peptide. Our study suggests a new way of utilizing biomolecule-conjugated AuNPs as potentially effective anticancer drugs.