Surface Modification of Magnetic Nanoparticle via Cu(I)-Catalyzed Alkyne-azide [2 + 3] Cycloaddition

详细信息    查看全文

• 作者：Po-Chiao Lin ; Shau-Hua Ueng ; Sheng-Chieh Yu ; Mi-Dan Jan ; Avijit Kumar Adak ; Ching-Ching Yu ; Chun-Cheng Lin
• 刊名：Organic Letters
• 出版年：2007
• 出版时间：May 24, 2007
• 年：2007
• 卷：9
• 期：11
• 页码：2131 - 2134
• 全文大小：183K
• 年卷期：v.9,no.11(May 24, 2007)
• ISSN：1523-7052

文摘

ournals/orlef7/9/i11/figures/ol070588fn00001.gif" ALIGN="left" HSPACE=5>

The Cu(I)-catalyzed alkyne-azide [2 + 3] cycloaddition has been demonstrated to be an effective and orthogonal conjugation reaction tocovalently immobilize biomolecules on magnetic nanoparticles (MNPs). The azido group on the MNP surface provides better conjugationefficiency with alkynated molecules. Moreover, the C-terminal alkynated protein was site-specifically immobilized on MNP. The protein bindingactivity presented by site-specific immobilization is higher than that by random amide bond formation.