Effect of Chitosan on Hepatic Drug-Metabolizing Enzymes and Oxidative Stress in Rats Fed Low- and High-Fat Diets

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• 作者：Hsien-Tsung Yao ; Chong-Kuei Lii ; Ruey-Hwang Chou ; Jia-Hsuan Lin ; Hui-Ting Yang ; Meng-Tsan Chiang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：April 28, 2010
• 年：2010
• 卷：58
• 期：8
• 页码：5187-5193
• 全文大小：742K
• 年卷期：v.58,no.8(April 28, 2010)
• ISSN：1520-5118

文摘

Chitosan is sold worldwide as a lipid-lowering functional food and may be taken with certain medications. To investigate the effect of chitosan on drug-metabolizing enzymes and oxidative stress in the liver, male Wistar rats were fed a low- or high-fat diet with cellulose or chitosan for 4 weeks. A significant decrease in cytochrome P450 (CYP) 3A-catalyzed testosterone 6β-hydroxylation in liver microsomes was observed in rats fed the chitosan with low- and high-fat diets. The expression of CYP 3A1 and 3A2, however, was suppressed by chitosan in rats fed the low-fat diet only. Furthermore, rats fed the low-fat diet with chitosan had lower hepatic glutathione S-transferase (GST) activity and superoxide dismutase activity and higher total tissue and microsomal lipid hydroperoxides. Hepatic α-tocopherol was lower in rats fed the chitosan-containing diet. The results suggest that chitosan is likely to modulate CYP 3A activity and protein expression and GST activity partially in a dietary fat-dependent manner. This change may cause a decrease in the metabolism of drugs catalyzed by these enzymes in liver tissues. Moreover, decrease of α-tocopherol level and SOD activity by chitosan partly accounts for the increase of hepatic lipid peroxidation.