Age-Dependent Modifications of the Human Salivary Secretory Protein Complex
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- 作者：Tiziana Cabras ; Elisabetta Pisano ; Roberto Boi ; Alessandra Olianas ; Barbara Manconi ; Rosanna Inzitari ; Chiara Fanali ; Bruno Giardina ; Massimo Castagnola ; Irene Messana
- 刊名：Journal of Proteome Research
- 出版年：2009
- 出版时间：August 7, 2009
- 年：2009
- 卷：8
- 期：8
- 页码：4126-4134
- 全文大小：296K
- 年卷期：v.8,no.8(August 7, 2009)
- ISSN：1535-3907
文摘
Physiological variability of the naturally occurring, human salivary secretory peptidome was studied as a function of age. The qualitative and quantitative changes occurring in the secretion of proteins/peptides specific to the oral cavity (i.e., basic salivary proline-rich proteins, salivary acidic proline-rich phosphoproteins, statherin, proline-rich peptide P-B, salivary cystatins, and histatins) were investigated by high-performance liquid chromatography-electrospray ionization-mass spectrometry in 67 subjects aged between 3 and 44 years. Subjects were divided into five age groups: group A, 8 donors, 3−5 years; group B, 11 donors, 6−9 years; group C, 20 donors, 10−12 years; group D, 15 donors, 13−17 years; group E, 13 donors, 24−44 years. Basic salivary proline-rich proteins, almost undetectable in the 3−5 and 6−9 years groups, reached salivary levels comparable to that of adults (24−44 years) around puberty. Levels of peptide P-D, basic peptide P−F, peptide P−H, peptide P−J (a new basic salivary proline-rich protein characterized in this study), and basic proline-rich peptide IB-1 were significantly higher in the 10−12-year-old group than in the 3−5-year-old group, whereas the increase of proline-rich peptide II-2 was significant only after the age of 12 years. The concentration of salivary acidic proline-rich phosphoproteins, histatin-3 1/24, histatin-3 1/25, and monophosphorylated and diphosphorylated cystatin S showed a minimum in the 6−9-year-old group. Finally, the histatin-1 concentration was significantly higher in the youngest subjects (3−5 years) than in the other groups.