Ling-Zhi Polysaccharides Potentiate Cytotoxic Effects of Anticancer Drugs against Drug-Resistant Urothelial Carcinoma Cells
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- 作者：Chao-Yuan Huang ; Jeff Yi-Fu Chen ; Jia-En Wu ; Yeong-Shiau Pu ; Guang-Yaw Liu ; Min-Hsiung Pan ; Ying-Tang Huang ; A-Mei Huang ; Chi-Ching Hwang ; Shu-Ju Chung ; Tzyh-Chyuan Hour
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2010
- 出版时间：August 11, 2010
- 年：2010
- 卷：58
- 期：15
- 页码：8798-8805
- 全文大小：1088K
- 年卷期：v.58,no.15(August 11, 2010)
- ISSN：1520-5118
文摘
The combined effects of ling-zhi polysaccharide fraction 3 (LZP-F3) and anticancer drugs (cisplatin and arsenic trioxide) were examined in three human urothelial carcinoma (UC) cells (parental, NTUB1; cisplatin-resistant, N/P(14); and arsenic-resistant, N/As(0.5)). MTT assay and median-effect analysis revealed that LZP-F3 could profoundly reverse the chemosensitivity of N/P(14) and N/As(0.5) to cisplatin and arsenic, respectively, in a dose-dependent manner, which involved activation of p38 and down-regulation of Akt and XPA. A dose of 10 μg/mL of LZP-F3 induced significant G1 arrest in N/P(14) and N/As(0.5) cells by flow cytometry, which may be mediated by the induction of p21WAF1/CIP1. The combination of LZP-F3 and arsenic trioxide produced a significant synergistic growth inhibition of NTUB1 and N/As(0.5) cells. Similar results were also found in N/P(14) cells. These molecular events of combined effects involved significant and earlier induction of Fas, caspase 3 and 8 activation, Bax and Bad up-regulation, Bcl-2 and Bcl-xL down-regulatuion, and cytochrome c release.