Enhancement of Natural Pigment Extraction Using Bacillus Species Xylanase
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- 作者：Dong Hyun Kim ; Jin Hee Kim ; Soon Ei Bae ; Jin Ho Seo ; Tae Kwang Oh ; and Choong Hwan Lee
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：April 6, 2005
- 年：2005
- 卷：53
- 期：7
- 页码：2541 - 2545
- 全文大小：83K
- 年卷期：v.53,no.7(April 6, 2005)
- ISSN：1520-5118
文摘
Pigment extracts from the root of Lithospermum erythrorhizon are used as natural red dyes, as wellas basic drugs due to their numerous pharmacological activities. In recent years, the demand forsuch natural pigment materials has increased; however, in natural dye production, the pigment yieldis strongly affected by the source of cultivation, extracting conditions, and solvents. Accordingly, thisstudy proposes a method of enzymatic pigment production based on the introduction of hydrolyticenzymes prior to the usual extraction to avoid repeated pigment extraction. The matrix destruction inthe epidermal layer of the root by the enzymes was found to improve the pigment extractability, thatis, the increment of KL, the mass transfer coefficient, representing the pigment mobility in the epidermallayer. The root tissue maceration by the hydrolytic enzymes was also measured to evaluate thepigment extractability, and a linear relationship was observed between the KL values and the tissuemaceration up to the addition of 3000 units/g of xylanase, indicating that the enzymatic macerationproportionally increases the interfacial area between the pigment and the solvent. Bacillus sp. DX107xylanase only served to increase the extractability of the pigment by loosening the root shell matrix,without affecting the contents and color properties of the pigment, as almost no difference was foundin the color between the pigments extracted using xylanase and those extracted according to thetraditional method.Keywords: Lithospermum erythrorhizon; xylanase; mass transfer coefficient; pigment extraction