文摘
Miniaturization of amperometric biosensors is cruciallydependent on the availability of methods for the nonmanual immobilization of biological recognition elementson the transducer surface. From an aqueous polymersuspension, the precipitation of a polymer film withentrapped biological recognition elements is initiated byelectrochemically induced oxidation of H2O at the electrode surface. Using the locally generated H+ gradient,acidic side chains of the polymer are titrated, leading toa change in the polymer solubility and hence to thecontrolled deposition of a polymer film. To investigate theproperties and limitations of this immobilization technology, the specific features of a glucose biosensor based onpolymer-entrapped glucose oxidase and amperometricdetection of enzymatically generated H2O2 were investigated. Besides the reproducibility of the immobilizationprocedure, the sensitivity (14.59 mA cm-2 M-1 at pH 7),long-term stability (up to 5000 measurements in asequential-injection analyzer), dependence on enzymeconcentration, polymer thickness, and possibilities tofabricate multilayer sensor architectures were exploited.In addition, the miniaturization potential of this nonmanual immobilization technology was evaluated by investigating the modification of microband electrode arraysand cross talk between the neighboring microsensors.