Cationic Two-Photon Lanthanide Bioprobes Able to Accumulate in Live Cells

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• 作者：Anh Thy Bui ; Maryline Beyler ; Yuan-Yuan Liao ; Alexei Grichine ; Alain Duperray ; Jean-Christophe Mulatier ; Boris Le Guennic ; Chantal Andraud ; Olivier Maury ; Raphaël Tripier
• 刊名：Inorganic Chemistry
• 出版年：2016
• 出版时间：July 18, 2016
• 年：2016
• 卷：55
• 期：14
• 页码：7020-7025
• 全文大小：397K
• 年卷期：0
• ISSN：1520-510X

文摘

An original cationic water-soluble cyclen-based Eu(III) complex [EuL¹]⁺ featuring a chromophore-functionalized antenna to increase the two-photon (2P) absorption properties was synthesized. The photophysical properties were thoroughly studied in various solvents and rationalized with the help of theoretical calculations. The complex exhibits an optimized 2P absorption cross section. Finally, 2P microscopy imaging experiments on living T24 human cancer cells highlighted the spontaneous internalization and the biological stability of this 2P bioprobe in vitro. Macrocyclic-based antennas open new perspectives for future optimization of the photophysical properties and allows envisaging the design of Eu, Tb, Yb, and Sm bioprobes. This result also opens the way for the design of functional two-photon Ln complexes able to monitor intracellular physicochemical parameters.