Bioresponsive Small Molecule Polyamines as Noncytotoxic Alternative to Polyethylenimine
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- 作者：Christopher R. Drake ; Abderrahim Aissaoui ; Orestis Argyros ; James M. Serginson ; Bryn D. Monnery ; Maya Thanou ; Joachim H. G. Steinke ; Andrew D. Miller
- 刊名：Molecular Pharmaceutics
- 出版年：2010
- 出版时间：December 6, 2010
- 年：2010
- 卷：7
- 期：6
- 页码：2040-2055
- 全文大小：497K
- 年卷期：v.7,no.6(December 6, 2010)
- ISSN：1543-8392
文摘
Nonviral gene therapy continues to require novel synthetic vectors to deliver therapeutic nucleic acids effectively and safely. The majority of synthetic nonviral vectors employed in clinical trials to date have been cationic liposomes; however, cationic polymers are attracting increasing attention. One of the few cationic polymers to enter clinical trials has been polyethylenimine (PEI); however, doubts remain over its cytotoxicity, and in addition it displays lower levels of transfection than viral systems. Herein, we report on the development of a series of small molecule analogues of PEI that are bioresponsive to the presence of pDNA, forming poly(disulfide)s that are capable of efficacious transfection with no associated toxicity. The most effective small molecule developed, a cyclic disulfide based upon a spermine backbone, is shown to form very well-defined polyplexes (100−200 nm in diameter) that mediate murine lung transfection in vivo to within an order of magnitude of in vivo jetPEI, and at the same time display a much improved cytotoxicity profile.