Comparing the Structural Topology of Integral and Peripheral Membrane Proteins Utilizing Electron Paramagnetic Resonance Spectroscopy

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• 作者：Daniel J. Mayo ; Johnson J. Inbaraj ; Nidhi Subbaraman ; Stuart M. Grosser ; Christopher A. Chan ; Gary A. Lorigan
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：July 30, 2008
• 年：2008
• 卷：130
• 期：30
• 页码：9656-9657
• 全文大小：146K
• 年卷期：v.130,no.30(July 30, 2008)
• ISSN：1520-5126

文摘

The alignment of membrane proteins provides pertinent structural and dynamic information. Structural topology data gleaned from such studies can be used to determine the functional mechanisms associated with a wide variety of integral membrane proteins. In this communication, we successfully demonstrate, for the first time, the determination of the structural topology and helical tilt of an antimicrobial peptide magainin 2 using aligned X-band spin-label EPR spectroscopic techniques. This novel comparison unlocks many possibilities utilizing EPR spectroscopy to probe antimicrobial peptide topologies with increased sensitivity and may also give further clues to elucidate their corresponding mechanisms.