Synthesis and Characterization of Oligonucleotides Containing a Nitrogen Mustard Formamidopyrimidine Monoadduct of Deoxyguanosine

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• 作者：Plamen P. Christov ; Kyu-Jun Son ; Carmelo J. Rizzo
• 刊名：Chemical Research in Toxicology
• 出版年：2014
• 出版时间：September 15, 2014
• 年：2014
• 卷：27
• 期：9
• 页码：1610-1618
• 全文大小：418K
• ISSN：1520-5010

文摘

N⁵-Substituted formamidopyrimidine adducts have been observed from the reaction of dGuo or DNA with aziridine containing electrophiles, including nitrogen mustards. However, the role of substituted Fapy-dGuo adducts in the biological response to nitrogen mustards and related species has not been extensively explored. We have developed chemistry for the site-specific synthesis of oligonucleotides containing an N⁵-nitrogen mustard Fapy-dGuo using the phosphoramidite approach. The lesion was found to be a good substrate for Escherichia coli endonuclease IV and formamidopyrimidine glycosylase.