Simple and Convenient G-Quadruplex-Based Turn-On Fluorescence Assay for 3鈥?鈫?5鈥?Exonuclease Activity

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• 作者：Chung-Hang Leung ; Daniel Shiu-Hin Chan ; Bradley Yat-Wah Man ; Chuan-Jen Wang ; Wing Lam ; Yung-Chi Cheng ; Wang-Fun Fong ; Wen-Luan Wendy Hsiao ; Dik-Lung Ma
• 刊名：Analytical Chemistry
• 出版年：2011
• 出版时间：January 15, 2011
• 年：2011
• 卷：83
• 期：2
• 页码：463-466
• 全文大小：173K
• 年卷期：v.83,no.2(January 15, 2011)
• ISSN：1520-6882

文摘

A selective, oligonucleotide-based, label-free, turn-on fluorescence detection method for 3鈥?鈫?5鈥?exonuclease activity has been developed using crystal violet as a G-quadruplex-binding probe. The assay is highly simple and rapid, does not require the use of gel-based equipment or radioisotopic labeling, and is amenable to high-throughput and real-time detection. A proof-of-concept of this assay has been demonstrated for prokaryotic Exonuclease III (ExoIII) and human TREX1.