Biochemical Basis for the Biological Clock

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• 作者：D. James Morré ; Pin-Ju Chueh ; Jake Pletcher ; Xiaoyu Tang ; Lian-Ying Wu ; and Dorothy M. Morré
• 刊名：Biochemistry
• 出版年：2002
• 出版时间：October 8, 2002
• 年：2002
• 卷：41
• 期：40
• 页码：11941 - 11945
• 全文大小：129K
• 年卷期：v.41,no.40(October 8, 2002)
• ISSN：1520-4995

文摘

NADH oxidases at the external surface of plant and animal cells (ECTO-NOX proteins) exhibitstable and recurring patterns of oscillations with potentially clock-related, entrainable, and temperature-compensated period lengths of 24 min. To determine if ECTO-NOX proteins might represent the ultradiantime keepers (pacemakers) of the biological clock, COS cells were transfected with cDNAs encodingtNOX proteins having a period length of 22 min or with C575A or C558A cysteine to alanine replacementshaving period lengths of 36 or 42 min. Here we demonstrate that such transfectants exhibited 22, 36, or40 to 42 h circadian patterns in the activity of glyceraldehyde-3-phosphate dehydrogenase, a commonclock-regulated protein, in addition to the endogenous 24 h circadian period length. The fact that theexpression of a single oscillatory ECTO-NOX protein determines the period length of a circadianbiochemical marker (60 X the ECTO-NOX period length) provides compelling evidence that ECTO-NOX proteins are the biochemical ultradian drivers of the cellular biological clock.